13585831301
021-59541103 021-60443211
3004967995
13585831301021-59541103 021-604432113004967995
在線訂購 免費訂購熱線：021-59541103 021-60443211
聯(lián)系人:高小姐
電話:021-59541103 021-60443211
手機:13585831301
Q Q： 3004967995
Email:3004967995@qq.com
詳細地址：上海嘉定區(qū)嘉羅公路1661
化學性質：
| 規(guī)格 | 10mg 25mg 100mg |
| CAS | 62669-70-9 |
| 別名 | R-22420,R-302,RH123 |
| 化學名 | 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]-xanthylium, monochloride |
| 分子式 | C21H17N2O3 Cl |
| 分子量 | 380.8 |
| 溶解度 | ≤10mg/ml in ethanol;10mg/ml in DMSO;10mg/ml in dimethyl formamide;10mg/ml in HCl(0.1M) |
| 儲存條件 | 4°C, protect from light |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice |
產品描述：
Rhodamine 123 is a fluorescent tracer dye used as substrate for P-glycoprotein.
Fluorescent tracer dyes represent a class of sub-cellular probes allowing the examination of cellular processes in real-time with minimal impact upon such processes. These tracer dyes are becoming increasingly used for the examination of membrane transport processes, since they are easy-to-use and cost effective probes for a number of membrane protein transporters, such as P-glycoprotein.
In vitro: Rhodamine 123, which was identified as a member of the rhodamine family of flurone dyes, was used to examine membrane transport by the ABCB1 gene product, MDR1. Previous study determined the λmax for excitation and emission for rhodamine 123 in commonly used solvents and extraction buffers, indicating that the fluorescence of rhodamine 123 was highly dependent on the chemical environment. The optimal parameters are 1% methanol in HBSS. In addition, the uptake of rhodamine 123 into cells was via both passive and active processes, and this occurred mainly by OATP1A2-mediated transport. Furthermore, this previous study quantified the intracellular sequestration and metabolism of rhodamine 123, showing that these were both cell line-dependent factors that might influence the interpretation of transport assays [1].
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原創(chuàng)作者：上海莼試生物技術有限公司
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賬 戶 名：上海生物
開 戶 行：中國銀行山東省分行營業(yè)部
賬 號：2169 2341 6278
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